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All the premium members are allowed to download prestar instantlyEffect of protein and glucose on insulin binding to cultured mouse hepatocytes.
Excess glucose is converted by the liver to glycogen and triacylglycerols, which normally protects against the development of insulin resistance. The present study examined the effect of the presence of excess glucose and/or proteins on the insulin binding capacity of isolated hepatocytes from normal C57BL/6 mice. With metabolic or treatment-induced insulin resistance, the specific insulin binding (10(6) sites/cell) on the isolated hepatocytes from normal C57BL/6 mice was approximately 40% of that on the corresponding control hepatocytes. The binding was saturable and of high affinity (Kd = 1.7 nM). The percentage increase in the binding was dose-dependent and nearly equal at 10 to 20 mM glucose (from basal) (from +31 to +63%). Protein (0.4-4 mg/ml) was assayed to determine its effect on the specific insulin binding. The binding was increased by 29-44% in the presence of 1-2 mg/ml of protein, and was decreased by 56% at 8 mg/ml of protein (from basal). In contrast, when the hepatocytes were cultured in media containing both 10 mM glucose and 0.4-8 mg/ml of protein, the decrease in the binding was much smaller (8-21%), suggesting a blunting of the decrease in insulin binding by proteins.If you are a metal fan, you probably remember this place, the Black Butte Cemetery. It is known to both musicians and non-musicians alike, thanks to the fact that it is located near Mt. Auburn, a small town on I-5 not far from the famous El Camino Real. There are a total of 17 footers as of this report, with four being tribute stones, and the rest being memorials.
Black Butte Cemetery is the resting place of some of the most notable people in the field of Heavy Metal. And not only does this place hold many history books with regards to the history of Heavy Metal
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