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Alonso was lapped by Hamilton for the first time in the team’s history as they plummeted from third to seventh in the championship in a bad day for Hamilton, who was also passed by Max Verstappen in a spectacular near-tragedy on lap six. The 2018 championship-winning driver’s hopes of another British Grand Prix success were ended by a spectacular episode of first-lap and then second-lap mayhem.
Hamilton, who had been in the top three until the final lap, was given a drive-through penalty for ignoring blue flags at the start, meaning he did not receive a penalty for speeding on the outside of the track. Alonso was subsequently passed by Hamilton’s team-mate and the two-time world champion took a slight dive down the order as he was seen to pull alongside the back of the Spaniard’s car.
“I really can’t believe that Lewis showed that kind of nerves [to pass me]” said Alonso. “After the whole season with speed and daring, it was a very big mistake of the 21-year-old Lewis to do that. I really hope he learns from that.”
“I can’t believe it, I didn’t expect it,” added Alonso. “All the races
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Resveratrol (Res) is known to exert chemopreventive and anti-inflammatory effects through a variety of mechanisms. The present study was conducted to investigate the effect and the underlying mechanism of Res in the regulation of MCF-7 cells proliferation. MCF-7 cells were treated with different concentrations of Res (2.5, 5, 10, and 20 μM) for different time periods (24, 48, and 72 hours), and the cell viability, reactive oxygen species (ROS) production, apoptosis, cell cycle and migration were measured by MTT assay, 2′,7′-dichlorofluorescein diacetate (DCFH-DA) assay, flow cytometry, and cell scratch assay, respectively. The phosphorylation of Akt and its substrate p70S6K1, as well as the expression of cell cycle proteins p21 and cyclin D1, were investigated by Western blotting. In addition, the effects of Res on ROS generation and the protective role of ROS scavengers were evaluated. The present study reveals that Res induces a dose-dependent decrease in MCF-7 cells viability, as measured by the MTT assay. Furthermore, the production of ROS is higher in the treated group than that in the control group. Res has been also found to induce the cell cycle arrest and migration in MCF-7 cells, and to up-regulate the expression of p21 and p-Akt in MCF-7 cells. In addition, resveratrol increased the levels of p-p70S6K1 and cyclin D1. Interestingly, the N-acetyl-L-cysteine (NAC) reduced the cell viability, ROS production, and cell cycle proteins expression induced by Res, as well as the phosphorylation of Akt and p-p70S6K1. This study indicates that Res induces cell cycle arrest and migration through the activation of p-p70S6K
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