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Sesquiterpene lactones and verbascoside, frequently co-occurring in medicinal plants, were separated in the presence of SDS, Triton X-100 and Brij-35 micelles, using micellar electrokinetic chromatography (MEKC), with direct UV detection at 225 nm. The effects of pH, BGE composition and buffer concentration on the retention behaviour of analytes were investigated. The running buffer containing Triton X-100 micelles (80 mM phosphate buffer, 30 mM SDS) under the chosen conditions (30 kV voltage, 20 mM borate buffer (pH 9.2)) showed the best separation of all analytes with 1.5% (v/v) of a surfactant (Brij-35). Concentrations as low as 500 microg ml(-1) for sesquiterpene lactones (beta- and gamma-patchoulol and alpha-humulene) and 2000 microg ml(-1) for verbascoside were detected under these conditions. The limits of detection were 0.1 microg ml(-1) (alpha-humulene), 0.5 microg ml(-1) (beta-patchoulol) and 10 microg ml(-1) (gamma-patchoulol) for all analytes. The method showed linear responses in the ranges of 250-2000 microg ml(-1) for alpha-humulene and beta-patchoul
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