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Laboratory and field studies have shown that non-pathogenic bacteria can produce the neurotransmitter acetylcholine (ACh) from a number of low-molecular-weight substrates. ACh is known to cause a distinctive intoxication syndrome in humans and rodents. To improve the understanding of cholinergic effects, we developed a field assay to quantify ACh, choline, phosphocholine, and ethanol in drinking water. The assay was calibrated using inoculation densities with Escherichia coli and Salmonella enterica Typhimurium. The ability of the bacteria to produce ACh in situ from phosphocholine was measured. Field assays were then performed in three replicate study areas. Ten millilitres of drinking water was consumed by 25 rats in each replicate area. The concentration of the analytes was measured in the rats’ faeces, which were transferred to the laboratory. Compared with E. coli and S. enterica Typhimurium, E. coli and S. enterica Typhimurium produced ACh from phosphocholine less efficiently. A non-monotonic effect was observed for inoculation density. Similar to previous studies, the measured concentrations of ACh were below the detection limit of the instrument. It is possible to apply the technique developed in this study to study drinking water samples with low cholinergic activity.It’s easy to fall into the trap of saying that every character in a show has to be perfect. This is a terrible way to judge a work as a whole. And yet we do it all the time.
For example, if you’ve ever watched an episode of the Wonder Showzen, or watched The Room, or seen the movie Clerks, or heard a The Simpsons song, then you know all too well what I mean. If you’ve watched even one episode of an anime, you’ve seen all these horrible characters that make you want to go throw something at the screen.
Here are some of the most common characters
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